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Video: Profiling Voltage-gated Potassium Channel mRNA Expression in Nigral Neurons using Single-cell RT-PCR Techniques
15.4K Views. University of Tennessee College of Medicine. The overall goal of this procedure is to detect messenger RNAs in individual neurons. This is accomplished by patch clamping and recording individual neurons then completely aspirate the cytoplasm from each recorded neuron from the Mr.mRNA and the cytoplasm. CD NA is made and amplified ...
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