CRISPR genome editing generates double-stranded breaks (DSBs) in genomic DNA and is a targeted method by which to achieve gene knockouts and knock-ins. Endogenous repair of DSBs results in an array of ...

Failed polymerase chain reactions (PCRs) are a common cause of frustration in the lab. While a typical PCR protocol is quite straightforward, scientists must optimize each step to amplify the intended ...

Designing reproducible PCR assays involves optimizing multiple moving targets, from standardizing each component in sometimes minute reaction volumes to planning ahead to ensure long-term and secure ...

The rapid spread of animal diseases and pathogen evolution have long posed significant threats to the healthy development of ...

Limited sample material and insufficient DNA input pose significant challenges for downstream analysis in various laboratory settings. To overcome this issue, isothermal amplification techniques have ...

Scientists from the United States have recently identified a novel approach to rapidly and accurately detect the omicron BA.2.12.1 sub-lineage of severe acute respiratory syndrome coronavirus 2 ...

Today, PCR is ubiquitous in molecular biology laboratories. As one of the first experiments that young scientists master, it is easy to take for granted. But developing this research lab staple ...

A new form of PCR has been used to improve the library preparation for forensic DNA typing from difficult samples like touch evidence and human remains. Typically, DNA sequencing and analysis is ...

Researchers at the Chan Zuckerberg Biohub, San Francisco, and collaborators have warned against only targeting one viral gene when carrying out reverse transcriptase-polymerase chain reaction (RT-PCR) ...