Fluorescence microscopy techniques are powerful tools for probing very small signals and revealing three-dimensional (3D) structural and functional properties of biological samples with high ...

The Abbe diffraction barrier, which fundamentally restricted the lateral optical resolution to 200 – 300 nm until the early 1990s, can be overcome by super-resolution optical fluorescence imaging ...

Nanoscopy is a field of microscopy that focuses on imaging and studying structures and processes at the nanoscale, typically below the diffraction limit of light. It encompasses various techniques ...

ISM is a super-resolution imaging technique that enhances resolution beyond the diffraction limit through pixel reassignment and deconvolution. However, the practical implementation of ISM requires ...