3D multiplexed imaging in cancer immunology enables a better characterization of tissue changes and cell interactions. Here, we introduce a one-shot, high-multiplexing workflow on the STELLARIS ...
Spatial carrier fringes in different directions, each recording the 3D information of the object at different time points, are captured and superposed through time integration within a single camera ...
Fig. 1 Schematic illustration of the DFAMFPP principle: Multiple dual-frequency fringe patterns are overlaid in a single long-exposure image. A deep neural network then reconstructs time-resolved 3D ...